Thu, 14 Dec 2017

VALIDATED RP-HPLC FOR SIMULTANEOUS ESTIMATION OF SITAGLIPTIN PHOSPHATE AND METFORMIN HYDROCHLORIDE IN TABLET DOSAGE FORM.

Shyamala.M*1, Mohideen.S1, Satyanarayana .T1, Ch.NarasimhaRaju1, Suresh Kumar.P1, Swetha.K1

 

1. Browns college of Pharmacy, Khammam, Andhra Pradesh.


ABSTRACT

Rapid and accurate High performance liquid chromatography method is described for Simultaneous estimation of Metformin Hydrochloride and Sitagliptin Phosphate from the combination tablet dosage form. The separation of two drugs was achieved on HYPERSIL (250 x 4mm i.d) 5μ column. The mobile phase consists of Acetonitrile and phosphate buffer in the ratio of 45:55. The detection was carried out at a wavelength 260nm. The method was validated for system suitability, linearity, accuracy, precision, robustness and stability of sample solution. The linear ranges for Metformin Hydrochloride and Sitagliptin Phosphate were 20-120μg/mL, 2-12μg/mL respectively with good recoveries i.e. 99.16% to 99.89%.

Keywords: Metformin hydrochloride, Sitagliptin phosphate, High performance liquid chromatography. 


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