Mon, 18 Dec 2017

In Vitro Hepatoprotective Activity of Limnanthemum Indicum [Whole Plant] Against Paracetamol Induced Toxicity Using Brl-3a Cell Line

Jyothi.Y1*, Sangeetha.D2, Kiran Kumar.K3

1Krupanidhi College of Pharmacy, Bangalore, India,

2Vellore Institute of Technology, Vellore, India

3Dr. Reddy’s Laboratories, Hyderabad, India


ABSTRACT

Liver plays a major role in detoxification. Any injury to it or impairment of its function may lead to many implications on one’s health. Management of liver diseases is still a challenge to modern medicine. The allopathic medicine has little to offer for the alleviation of hepatic ailments whereas the most important representatives are of phytoconstituents. The work presented in this paper is on plant Limnanthemum indicum which is reported that the whole plant is traditionally used as hepatoprotective. The study was aimed at evaluation of the hepatoprotective activity of the whole plant of Limnanthemum indicum on the BRL3A (Rat, Liver cell line) cell line. The various solvent extracts were tested for its inhibitory effect on BRL3A (Rat, Liver cell line) cell line. The percentage viability of the cell line was carried out. The cytotoxicity of Limnanthemum indicum on rat liver cell was evaluated by the MTT assay [(3-(4,5 dimethylthiazole –2 yl)-2,5 diphenyl tetrazolium bromide) assay]. The principle involved is the cleavage of tetrazolium salt MTT into a blue coloured derivative by living cells which contains mitochondrial enzyme succinate dehydrogenase However, the information available on the pharmacological activity of the plant is very limited. Hence, it was proposed to carry out a preliminary in vitro analysis of the hepato protective activity of the plant. Out of the various extracts evaluated, ethanolic extract of Limnanthemum indicum gave promising results.

Keywords: Hepatoprotective, Rat liver cell line, MTT, Mitochondrial


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