DOI: 10.21276/ajptr
Sat, 25 May 2019

A Validated RP-HPLC Method for the Determination of Linagliptin


Lakshman Raju Badugu

1. Department of Chemistry, Bhagwan Sri Satya Saibaba PG College, Tadikonda, A.P, India




Linagliptin frequently associated in pharmaceutical a formulation that reduces blood sugar levels in patients with type 2 diabetes. Their quantification presents several problems. A HPLC method for the determination of these compounds in pharmaceutical formulations, including the separation of impurities and excipients has been developed and validated. The method was simple, selective, linear, precise and accurate. Isocratic elution at a flow rate of 1ml min-1 was employed on a symmetry C18 column at ambient temperature. The mobile phase consisted of Methanol: Water 83:17(v/v) and pH of the mobile phase was adjusted to 4.1 with 0.1% Orthophosphoric Acid . The UV detection wavelength was at 241nm.Linearity was observed in concentration range of 5-30ppm. The retention time for Linagliptin was 5.85min.The method was validated as per the ICH guidelines. The proposed method can be successfully applied for the estimation of Linagliptin in pharmaceutical dosage forms.

Key Words: Linagliptin, HPLC Development and Validation, ICH guidelines.

[PDF]   Viewed: 7810   Downloaded: 1329
  • Call for paper

    American Journal of PharmTech Research (AJPTR) is a peer-reviewed, bimonthly official international journal allowing access to abstracts and full-text.



  • Email & SMS Alerts

    Email/SMS alert system is one of the key features and gives update information about the submitted manuscript.


  • Article Statastics

    American Journal of PharmTech Research (AJPTR) provides unique facility for authors to know popularity of articles on basis of numbers of PDF download and views. It will display in tabulated format.

  • Online Submission

    American Journal of PharmTech Research (AJPTR) is one of the International open access journal devoted to various disciplines in science and technology.


web counter
web counter