Fri, 25 Apr 2014

Development and Validation of RP-HPLC Method for Simultaneous Estimation of Levocetirizine Dihydrochloride and Phenylephrine in Bulk and In Tablet Dosage Form

 

Deepali S. Tuljapure1*, Narendra M. Gowekar1, Savita S.Yadav1, Aashish S. Mogale1

1. Department of Pharmaceutical Chemistry, Sinhgad Institute of Pharmaceutical Sciences, Lonavala -410401, Maharashtra, INDIA

2. Bharti Vidyapeeths’ Poona College of Pharmacy,Erandwane,Pune-38

3. Sahyadri college of Pharmacy Methwade. Sangola Dist. Solapur


 

ABSTRACT

 

The present work deals with development and validation for simultaneous determination of antihistaminic drugs in pharmaceutical formulations. A rapid, precise and specific high performance liquid chromatography (RP-HPLC) method was developed for Levocetirizine dihydrochloride and Phenylephrine. Chromatographic separations was achieved on Waters Younglin system C-18 (5μm, 250×4.6 mm) HPLC column within a short runtime of 10 min. HPLC system having isocratic mode, with mobile phase containing methanol : water (pH 3) (70:30% v/v) and flow rate maintained at 1.0 mL/min was used. Effluents were monitored at 230 nm. Retention time of Levocetirizine dihydrochloride and Phenylephrine were found to be 2.6 and 4.6 min respectively. Linearity was studied in the concentration range of 2 to 12 μg/mL and 12 to 72 μg/ mL for Levocetirizine dihydrochloride and Phenylephrine respectively, with a correlation coefficient of 0.998 and 0.999 respectively. The proposed method was validated according to the ICH guidelines with respect to specificity, linearity, accuracy, precision and robustness.

Keywords: RP-HPLC, Levocetirizine dihydrochloride, Phenylephrine, Specificity, Validation


[PDF]  Viewed: 893  Downloaded: 208
  • Call for paper

    American Journal of PharmTech Research (AJPTR) is a peer-reviewed, bimonthly official international journal allowing access to abstracts and full-text.

    Read more...

  • Email & SMS Alerts

    Email/SMS alert system is one of the key features and gives update information about the submitted manuscript.

    Read more...

  • Article Statastics

    American Journal of PharmTech Research (AJPTR) provides unique facility for authors to know popularity of articles on basis of numbers of PDF download and views. It will display in tabulated format.

  • Online Submission

    American Journal of PharmTech Research (AJPTR) is one of the International open access journal devoted to various disciplines in science and technology.

    Read more...

web counter
web counter