Sat, 1 Nov 2014

Qualitative Analysis of Serum Proteins in Oral Cancer Separated by SDS-PAGE

 

Nishant Sharma1*, B.R Shrivastav2, Archana Shrivastav2

1.  Department of Biotechnology, College of Life Sciences, Cancer Hospital and Research Institute, Gwalior, MP, India.

2.  College of Life Sciences, Gwalior, MP, India.


 ABSTRACT

 

This study was conducted for qualitative estimation of serum proteins separated by SDS-PAGE in order to describe the definition of potential serum proteins that may act as diagnostic marker in oral cancer. Serum samples of 25 biopsy confirmed cases of oral cancer and normal controls of similar age group were subjected to SDS-PAGE on a 12% resolving gel, followed by staining with Coomassie Brilliant Blue R-250. Protein fractions were analyzed using computer software program “Gene Genius Gel Documentation and Analysis System”. Major protein fractions ranging in molecular weights from 1.46-159 kDa were observed. Raw volumes of most of the protein fractions seem to be increased in majority of oral cancer cases as compared to normal control. Protein fractions 56-58 kDa were undetected in normal controls under 80 years of age but appeared in 54% of Oral cancer patient. Further advancement of this work could yield better resolution of protein fraction 56-58 kDa that could serve as marker for oral cancer.

Keywords: oral cancer, serum protein, analysis, SDS-PAGE.


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