DOI: 10.21276/ajptr
Sat, 25 May 2019

Development and Validation of Dual Wavelength Method for Simultaneous Estimation of Nebivolol Hydrochloride and Hydrochlorothiazide In Tablet Dosage Form


Megha D. Madhekar*1, Narendra M. Gowekar1, Kedareshwar G. Jadhav1, Darshali S. Desai1, Pooja P. Dhanawade1, Savita N. Gowekar2.

1. Department of Pharmaceutical Chemistry, Singhad institute of pharmaceutical sciences, Pune   University, Lonavala(Pune), Maharashtra, INDIA.

2. BVU Poona Collage of Pharmacy, Erandwane, Pune, Maharashtra, India




The present work describe simple, sensitive, rapid, accurate, precise and economic dual wavelength spectrophotometric method for the simultaneous estimation of Nebivolol hydrochloride and Hydrochlorothiazide in combined tablet dosage form. The principle for dual wavelength method is “the absorbance difference between two points on the mixture spectra is directly proportional to the concentration of component of interest”. The utility of this method is its ability to calculate unknown concentration of components of interest in a mixture containing an interfering component. The method was based on determination of Nebivolol hydrochloride at 246 nm and 292 nm and Hydrochlorothiazide at 264 nm and 295 nm. The two drugs follow Beer’s law over the concentration range of 5-30 µg/ml. The method was successfully applied to pharmaceutical dosage form. The results of analysis have been validated as per ICH guidelines.

Keywords: Nebivolol hydrochloride, Hydochlorothiazide, Dual wavelength spectrophotometric method, Validation.

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