DOI: 10.21276/ajptr
Mon, 27 May 2019

Estimation of Ranolazine Using Reverse Phase High Performance Liquid Chromatography Technique


Boovizhikannan Thangabalan*1,2, Palanirajan Vijayaraj kumar3

1.Department of Pharmaceutical Analysis, Southern institute of medical sciences, College of Pharmacy, Mangaldas Nagar, Guntur-522 001, Andhra Pradesh, India.

2.Department of Pharmaceutical Sciences, Jawaharlal Nehru Technological University Hyderabad, Kukatpally, Hyderabad - 500 085, Andhra Pradesh, India.

3.Faculty of Pharmaceutical sciences, School of pharmacy, UCSI (University college of Sadaya International) University, Malaysia.



A simple, precise, rapid and accurate reverse phase high performance liquid chromatography method (RP-HPLC) in isocratic mode has been developed for the estimation of ranolazine in pure form and in its tablet dosage form. An Agilent Eclipse XDB C18, 150 x 4.6 mm, 5mm particle size column, with mobile phase consisting of phosphate buffer pH 3.5 and acetonitrile in the ratio of 65:35 % v/v was used. The flow rate was 1.0 mL/min and the column effluent was monitored at 272 nm. The retention time was 4.7 min. The detector response was linear for ranolazine in the concentration range of 10-150 µg/mL. The limit of detection (LOD) was found to be 0.034 μg/mL. The limit of quantification (LOQ) was 0.102 μg/mL. The method was validated by determining its accuracy, precision and system suitability. The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate, which is useful for the routine determination of ranolazine in pure drug and in its tablet dosage form.

Keywords: Ranolazine, RP-HPLC estimation, tablets.

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