DOI: 10.21276/ajptr
Mon, 25 Mar 2019

RP-HPLC Method Development and Validation for Nimorazole


Arun M. Kashid*1, Navin S. Dawra1, Anup A. Dhange1, Afroj I. Mulani1, Deepali A. Ghorpade1, Shashikant C. Dhawale2

1.Department of Pharmaceutical Chemistry, Sinhgad Institute of Pharmacy, Pune-411041, Maharashtra, India

2.Department of Pharmacology, School of Pharmacy, SRTM University, Nanded- 431606 Maharashtra, India.



A new reverse phase high performance liquid chromatography (RP-HPLC) method for the quantitative determination of Nimorazole was developed and validated as per ICH guidelines. The analyte was injected into an HIBER C18 column (150 mm × 4.6 mm, 5μm), maintained at ambient temperature and effluent was monitored at 297 nm. The mobile phase consisting of acetonitrile: methanol: buffer (2:3:5 v/v/v). The pH of the mobile phase was adjusted to 4.0 by using O-phosphoric acid. The flow rate was maintained at 1.0 mL/min. and retention time was observed 1.76 min. The developed method shows high specificity for Nimorazole. Calibration curve was plotted with a range from 1-5µg/ml (r2>0.999). The lower limit of quantification (LLOQ) was found to be 0.5μg/ml. The method was validated for parameters like accuracy, precision, recovery, linearity, robustness. This RP-HPLC method is suitable for determining the concentration of Nimorazole and it was applied to routine analysis for determination of the Nimorazole from its formulation during pharmacokinetic study.

Keywords: Nimorazole, RP-HPLC, Validation.

[PDF]   Viewed: 2337   Downloaded: 280
  • Call for paper

    American Journal of PharmTech Research (AJPTR) is a peer-reviewed, bimonthly official international journal allowing access to abstracts and full-text.



  • Email & SMS Alerts

    Email/SMS alert system is one of the key features and gives update information about the submitted manuscript.


  • Article Statastics

    American Journal of PharmTech Research (AJPTR) provides unique facility for authors to know popularity of articles on basis of numbers of PDF download and views. It will display in tabulated format.

  • Online Submission

    American Journal of PharmTech Research (AJPTR) is one of the International open access journal devoted to various disciplines in science and technology.


web counter
web counter