Tue, 19 Dec 2017

Enzymatic Extraction of Curcumin from Curcuma Longa Rhizome

 

Saroj Yadav1*, Anoop Kumar2

1*Mallige College of Pharmacy, Bangalore, India

2. Dept. of Pharmaceutical Technology, MEIT Meerut, India


 

ABSTRACT

The selective enzyme assisted extraction of curcumin was developed and optimized from Curcuma longa. When water was used as solvent, 3% of curcumin were extracted representing a search for an interesting alternative for the extraction in industrial processes. A selective extraction process after the treatment with enzymes is proposed by using 30% (v/v) methanol which releases up to 15% of the curcumin, present in the rhizome. The optimal conditions were as follows: pH value was 5.5, concentration of cellulase solution was 2.5 mg/mL, incubation time was 8 h, incubation temperature was 50 ºC and solid:solvent ratio was 1:8 . Enzyme-assisted extraction was proved to be environment-friendly and economical, and could be used in natural product extraction in large scale.

Keywords: Curcuma longa, Total curcuminoids, enzymatic extraction


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