Sun, 17 Dec 2017

Simultaneous Quantification of Umbelliferone and Quercetin in Polyherbal Formulations of Aegle Marmelos by HPTLC.

 

Nadeem A. Siddique1, Mohd. Mujeeb1, Mohd Aamir1, Asif Husain2*

 

1. Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Jamia Hamdard, New Delhi, India.

2. Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Jamia Hamdard, New Delhi, India.


 

ABSTRACT

In the present investigations, methanolic extracts of four marketed Aegle marmelos formulations (F1, F2, F3, and F4) were prepared and subjected to simultaneous quantitative determination of two biologically active compounds; umbelliferone and quercetin. Analysis of umbelliferone and quercetin was performed on TLC aluminum plates pre-coated with silica gel 60F-254 as stationary phase. Linear ascending development was carried out in twin trough glass chamber saturated with mobile phase consisting of toluene: ethyl acetate: formic acid (6:4:1, v/v/v), and densitometric determination of these compounds was carried out at 300nm in reflectance/absorbance mode. The system was found to give compact spots for umbelliferone and quercetin with Rf value of 0.66 and 0.68, respectively. The present method was validated for precision, recovery, repeatability, and accuracy in accordance with International Conference on Harmonisation (ICH Q2) guidelines. Statistical analysis of the data showed that the method is reproducible and selective for estimation of umbelliferone and quercetin. This method may be used for routine quality control and standardization of the herbal drugs and there formulations.

Key words: Aegle marmelos, Quercetin, Umbelliferone, HPTLC

 


[PDF]  Viewed: 1356  Downloaded: 170
  • Call for paper

    American Journal of PharmTech Research (AJPTR) is a peer-reviewed, bimonthly official international journal allowing access to abstracts and full-text.

    Read more...

     

  • Email & SMS Alerts

    Email/SMS alert system is one of the key features and gives update information about the submitted manuscript.

    Read more...

  • Article Statastics

    American Journal of PharmTech Research (AJPTR) provides unique facility for authors to know popularity of articles on basis of numbers of PDF download and views. It will display in tabulated format.

  • Online Submission

    American Journal of PharmTech Research (AJPTR) is one of the International open access journal devoted to various disciplines in science and technology.

    Read more...

web counter
web counter