Sun, 17 Dec 2017

Determination of total Phenolic and Flavanoid Contents in Traditionally used Aegle Marmelos Formulations by Spectrophotometric Method

 

Nadeem A. Siddique1*, Mohd. Mujeeb1, Sayeed Ahmad1, Asif Husain2

 

1. Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Jamia Hamdard (Hamdard University) New Delhi-110062, India

2. Department of Pharmaceutical chemistry, Faculty of Pharmacy, Jamia Hamdard (Hamdard University) New Delhi-110062, India


 

ABSTRACT

In present investigation, methanolic extract of different marketed Aegle marmelos formulations (F1, F2, F3, and F4) screened for determination of total phenolic and flavanoid contents.  Highest phenolic (6.145 ± 0.05 mg/kg) and flavonoid (8.134 ± 0.044 mg/kg) contents were noticed in formulation F4. Free radical scavenging activity of all the studied formulations were evaluated by using DPPH (1,1-Diphenyl-2-picryl hydrazyl) method. Whereas, each formulation showed good scavenging of DPPH radical with IC50 values (F1-2.185, F2-2.216, F3-2.243 and F4-2.143 µg/ml), and is comparable to standard compounds (BHT, ascorbic acid and rutin).

Key-Words: Aegle marmelos, Total phenolic contents, Total flavanoid contents, DPPH

 


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