DOI: 10.21276/ajptr
Sun, 21 Apr 2019

Phytochemical screening and isolation of a pure phytoconstituent from the leaves of Callistemon salignus


Sailee Chowdhury 1*, Ashoke Kumar Ghosh 2, Sushomasri Maji,3, Nripendra Nath Bala 1


1. BCDA College of Pharmacy & Technology, Barasat, Kolkata -700 127, India.

2. IFTM University , Moradabad- 244102, Uttar Pradesh, India

3. Bengal School of Technology, Hoogly – 712102, India



The methanol extract of Callistemon salignus was subjected to different phytochemical tests  and gives positive results for glycosides, tannin, carbohydrate, volatile oil, flavonoid and steroid. This extract leads to the isolation of 3′, 4′, 5, 7-tetra-hydroxy flavonol by chromatographic separation through a graded elution. The identity of the isolated compound was checked by preliminary phytochemical test, TLC study, solubility, melting point determination. Finally the structure was elucidated by different spectroscopic methods such as UV, IR, NMR (13 C NMR and Proton NMR) and LCMS. On the basis of this chemical and spectral evidence and upon comparison with the literature data, the isolated compound is identified .The compound was isolated first time from this plant extract.

Keywords:Callistemon salignus, 3′, 4′, 5, 7-tetra-hydroxy flavonol, graded elution, spectroscopic methods.


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