Sat, 18 Nov 2017

Effect of Catecholamine on Indomethacin-Induced Ulceration and Apoptosis in Ratís Stomach

 

Olorunfemi O. Joyce*1, Nworah D. Chinwe1, Egwurugwu J. Nnabufe2, Woyike A. Ovutor.1, Adienbo O. Macstephen1.

1.Department of Human Physiology, Faculty of Basic Medical Sciences, College of Health Sciences, University of Port Harcourt, Rivers State, Nigeria.

2.Department of Human Physiology, College of Medicine & Health Sciences, Imo State University, P.M.B. 2000, Owerri, Imo State, Nigeria.


 

ABSTRACT

In the present study, the effects of pre-treatment with catecholamine on indomethacin-induced ulcer were studied. Rats were exposed to various treatments with epinephrine and dopamine 30 minutes before ulcer was induced using NSAID (indomethacin). Experimental ulceration was induced in fasted rats using Indomethacin (40mg/kg.p.o). Four hours later after indomethacin administration, the stomachs were opened under thiopentane anesthesia and the ulcer area scored by planimetry. Sections of the stomachs were prepared for histology and stained for apoptotic cell count. Acid secretion was also studied in the control and treated animals by pylorus ligation technique. Indomethacin treatment resulted in the formation of ulcer with ulcer index of 5.0±0.5 while the pre-treatment with catecholamine significantly reduced ulceration episodes (epinephrine: ulcer index=3.0±0.7, dopamine: ulcer index=2.0±0.7, p<0.05). Total Apoptotic score in the indomethacin-induced rats’ stomachs was 19.5±0.38. This was reduced to 4.3±1.70 and 1.3±0.33 in the epinephrine and dopamine treated rats respectively. Administration of catecholamine prior to indomethacin resulted in a significant reduction in total stomach acidity from 2.381±0.042 (indomethacin alone) to 1.581±0.12 (epinephrine-treated) and 1.358±0.12 (dopamine-treated) p<0.05. However, dopamine was found to have more inhibitory influence compared to epinephrine. The results suggest an effective drug combination therapy clinically for ulcer patients placed on NSAID especially indomethacin.

Keyword: Ulcer, catecholamine, apoptosis, total acid output, indomethacin, pylorus ligation technique.


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