DOI: 10.21276/ajptr
Mon, 27 May 2019

Spectrophotometric Determination of Moxifloxacin HCl in Pure and Blood Sample

Arshad Mahmood1*, Asif Hanif Chaudhry2, Kazi Muhammad Ashfaq3, Tanveer Akhtar Malik3, Rashid Mahmood3

1.Department of Chemistry, Umea University, Sweden.

2.GARL, GSP, Islamabad, Pakistan

3.National Institute of Health, Islamabad, Pakistan


A reaction between Moxifloxacin HCl and FeCl3 solution in slightly acidic medium was studied. It gave brown colour complex having maximum absorbance at 407.0nm. The reaction is selective for Moxifloxacin HCl with 0.00024µg/uL and different concentration of FeCl3 was applied.  The color reaction obeyed Beer’s law from 0.01 µg /µL to 0.00018 µg/µL of FeCl3 solution having concentration 0.1ug∕uL and relative standard deviation 0.026%.The quantitative estimation of moxifloxacin HCl in blood sample is also studied.

Key Words: Moxifloxacin, UV-Visible Spectrophotometer, Ferric Chloride, Beer’s law, Color reaction, Analytical method

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