DOI: 10.21276/ajptr
Mon, 27 May 2019

A Validated RP-HPLC Method for the Simultaneous Estimation of Dextromethorphan Hydrobromide and Chlorpheniramine Maleate in Syrup Formulation


Krushna D. Khalode*1, Shekhar B. Waikar1, Suhas P. Padmane1

1. Gurunanak College of Pharmacy, Nari, Kamptee road, Nagpur 440026, Maharashtra, India



The proposed method is a simple, accurate, precise, specific and rapid method for the simultaneous estimation of dextromethorphan hydrobromide (DXM) and chlorpheniramine maleate (CPM) in bulk and syrup formulation. Stationary phase consist of Eclipse-XDB C18 column(150×4.6mm, 5μm) and mobile phase with gradient mode consisting of phosphate buffer (adjusted to pH 3.0 with o-phosphoric acid): acetonitrile (80:20 v/v) was used. The flow rate was set at 1.0 ml/min and UV detection was carried out at 272 nm. The retention time of DXM and CPM were 9.05 min and 7.53 min respectively. The % recovery of DXM and CPM was found to be 99.58 ±1.33 and 98.24 ±1.97 respectively. DXM and CPM drugs were found to be linear over the concentration range of 2-50 µg/ml and 0.8 - 20 µg/ml respectively. The proposed method can be useful in the quality control of DXM and CPM in bulk drug and drug products. 

Keywords: Chlorpheniramine maleate, Dextromethorphan hydrobromide, RP-HPLC, Validation.

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