Mon, 18 Dec 2017

The Study of Biochemical Activity and DNA Extraction from Euphorbia hirta

 

I. Arul Prakash1, S.Ayyanar1, N.Mohamed Irfanudeen1, V.Vadana Sundari1*

1.Department of bio-technology, P.R. Engineering college, Tanjore, Tamil Nadu, India


 

ABSTRACT

Euphorbia hirta is native to India but is a pan tropical weed. It is used in traditional medicine for the treatment of boils, wounds and control of diarrhoea and dysentery. Therefore the Crude  from different parts (leaf, and stem) of Euphorbia hirta (Euphorbiaceae) were extracted by different solvents   and screened the antimicrobial activity by disc diffusion assay against five bacteria and 2 Fungi (Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi , Bacillus subtilis, Klebsiella pnemoniae & Aspergillus Niger, Aspergillus flavus). The Minimum inhibitory concentration (MIC), Minimum bactericidal concentration (MBC) of stem and leaves extracts against each sensitive has also been evaluated. The maximum effect has shown in Pseudomonas aeruginosa (7mm in leaf and 6 mm in stem). The leaf is having higher effect than stem. The basic photochemical analysis showed the presence of biologically active compounds in stem and leaf of E.hirta. The minimum effect of extract observed by soxhlet extraction Pseudomonas aeruginosa (5mm in leaf and 3 mm in stem). The Alkaloids extracted  by standard method and its activity also measured Pseudomonas aeruginosa (0.5mm in leaf and 0.6 mm in stem) , Then the crude, alkaloid & pure component of the plant parts (leaf, and stem) is compared. The crude, pure and alkaloids of leaves has higher inhibition than stem. The alkaloid is lower inhibition than crude and pure. In the two part of inhibiting activity Pseudomonas aeruginosa has the maximum effect. In alkaloid inhibiting activity Salmonella typhi has the maximum effect. DNA extracted from the leaf of the plant. 


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