Mon, 20 Nov 2017

Isolation and Screening of L - Asparaginase Producing Marine Actinomycetes from South Indian Coastal Region

K.Selvam1*, B. Vishnupriya1

1. Dr .N.G.P Arts and Science College, Affiliated to Bharathiar University, Coimbatore - 48, Tamilnadu.


ABSTRACT

A total of fifty six actinomycete isolates were isolated from the ten marine sediments of south India. Marine environment is a potential source of novel actinomycetes, which are a potent source of antibiotics and novel bioactive compounds. The isolates were identified as actinomycetes by morphological, biochemical and microscopic studies. The isolated actinomycetes were screened for L-asparaginase activity by rapid plate assay method. Based on screening, isolate 1, 2 and 18 were showed large clear pink zone and its diameter (dm) was measured as 9.0, 8.5 and 8.5 cm and the enzyme production has been determined by nesslerization method. The spectrophotometric assay of enzyme activity of the isolates 1, 2 and 18 were found to be 1.92, 1.48 and 1.46 U/ml.

Keywords: L-asparaginase, Marine actinomycetes, Nesslerization, Semi quantitative plate assay


[PDF]  Viewed: 1392  Downloaded: 214
  • Call for paper

    American Journal of PharmTech Research (AJPTR) is a peer-reviewed, bimonthly official international journal allowing access to abstracts and full-text.

    Read more...

     

  • Email & SMS Alerts

    Email/SMS alert system is one of the key features and gives update information about the submitted manuscript.

    Read more...

  • Article Statastics

    American Journal of PharmTech Research (AJPTR) provides unique facility for authors to know popularity of articles on basis of numbers of PDF download and views. It will display in tabulated format.

  • Online Submission

    American Journal of PharmTech Research (AJPTR) is one of the International open access journal devoted to various disciplines in science and technology.

    Read more...

web counter
web counter