DOI: 10.21276/ajptr
Tue, 21 May 2019

Method Development and Validation for Quantification of Betaine in Achyranthes aspera by HPLC

VB Krishna1, Naira Nayeem1*

1.Krupanidhi College of Pharmacy, Bangalore.


A simple, accurate, rapid and precise isocratic High performance liquid chromatographic (HPLC) method was developed and validated for the quantification of betaine in Achyranthes aspera. The method employs Reversed Phase Phenomenex (US) C18 Column 250 x 4.6mm I.D, 5µ Particle Size system consisted of prominence LC-20AT gradient pumps, Prominence SIL- 20A Auto sampler and a Prominence SPD-M20A Diode Array Detector (SHIMADZU), Japan and flow rate of 1.0 ml/min with a load of 20μl. Water and acetonitrile was used as mobile phase in the composition of 10:90. The detection was carried out at 242 nm. Linearity range was 0.32-0.48 mg/ml. Retention time of betaine was found to 8.42 min. The recovery studies 98-103%. This method was validated for accuracy, precision, linearity and Robustness as per ICH guidelines. The method was found to be specific, selective, and robust.

Keywords: Achyranthes aspera, Betaine, RP HPLC, validation

[PDF]   Viewed: 3352   Downloaded: 401
  • Call for paper

    American Journal of PharmTech Research (AJPTR) is a peer-reviewed, bimonthly official international journal allowing access to abstracts and full-text.



  • Email & SMS Alerts

    Email/SMS alert system is one of the key features and gives update information about the submitted manuscript.


  • Article Statastics

    American Journal of PharmTech Research (AJPTR) provides unique facility for authors to know popularity of articles on basis of numbers of PDF download and views. It will display in tabulated format.

  • Online Submission

    American Journal of PharmTech Research (AJPTR) is one of the International open access journal devoted to various disciplines in science and technology.


web counter
web counter