Mon, 20 Nov 2017

Identification of Bioactive Compounds from Spirulina by Gas Chromatography Coupled with Mass Spectrophotometer (GC-MS)

Vasanth Ramasamy1, Velliyur Kanniappan Gopalakrishnan1*

1.Department of Biochemistry and Bioinformatics, Karpagam University, Coimbatore – 641 021 Tamil Nadu, India


ABSTRACT

The aim of the present study was to investigate the chemical composition of Spirulina subjected to different solvent extraction (acetone, methanol and ethyl acetate) by using soxhlet methodology and the extracts were analysis by Gas chromatography coupled with mass spectrophotometer (GC-MS) using DB-5 capillary column. During GC-MS analysis, it was observed that fatty acid components are present in their extracts. In acetone extract, totally forty compounds were identified and heptacosane (17.25%), hexacosane (17.04%), heneicosane (14.47%), pentacosane (13.22%) and Nonacosane (11.29%) as major components. Thirty five compounds were identified in methanol extract and the major compounds are n-Hexadecanoic acid (19.9%), Cyclononasiloxane octadeca methyl (7.81%) and phytol (6.53%). Thirty components were identified in ethyl acetate extract and dodecane (17.35%), heptadecane (15.31%), sufurous acid butyl heptadecyl ester (11.31%) and n-hexadecanoic acid (4.26%) most abundant in ethyl acetate extract.

Keywords: Spirulina, GC-MS, acetone, methanol, ethyl acetate


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