Mon, 20 Nov 2017

Hepatic Targeting of Conconavalin-A Appended Myristoyl Chitosan Nanoparticles Containing Epirubicin

Pooja Sharma1,Rayasa Ramachandra Murthy*1

1.Nanomedicine Research Centre, I.S.F College of Pharmacy, Moga, Punjab- 142001


ABSTRACT

Hepatic targeting of Concanavalin A appended myristoyl chitosan nanoparticles containing Epirubicin. Myristoyl chitosan was synthesised by reacting native chitosan with myristoyl chloride and  degree of acylation was determined by Ninhydrin assay. Nanoparticles of chitosan and myristoyl chitosan were prepared by ionic gelation and the method was optimised for processing parameters based on particle size, zeta potential and entrapment efficiency (EE). The nanoparticles of chitosan and myristoyl chitosan were conjugated with concanavalin A by incubation and the conjugation was confirmed by zeta potential measurement. The surface morphology of the optimized formulation was checked with the help of SEM and was further studied for organ distribution studies in Wistar rat model. Myristoyl chitosan synthesised was confirmed by FT-IR studies. Degree of acylation was found out to be 42.2 ± 2.7%. The optimized Con A conjugated nanoparticles prepared by chitosan (Ch17) and myristoyl chitosan (MCh17) was found to be spherical in shape with particle size 244.4nm and 275.8nm, zeta potential of 0.307mV and 0.133mV, entrapment efficiency 45.01±1.32% and 40.10±1.23% respectively. In vitro drug release (PBS 7.4) from Ch17 was 93.02±1.66% and followed Higuchi model, while release from MCh17 was 68.53±2.27% and followed Peppas model. Both the formulation were stable for 1 month at the temperature of 2-8oC. In vivo liver uptake of MCh17 nanoparticles was 93.6±10.11% while it was  87.0±7.55% with Ch17. Epirubicin loaded MCH17 nanoparticle showed high uptake by liver with concomitant reduction in blood level of Epirubicin in comparison to  Ch17 nanoparticles.

Keywords: Chitosan, Concanavalin A, Epirubicin, Liver targeting, Myristoyl chitosan, Nanoparticles.


[PDF]  Viewed: 931  Downloaded: 94
  • Call for paper

    American Journal of PharmTech Research (AJPTR) is a peer-reviewed, bimonthly official international journal allowing access to abstracts and full-text.

    Read more...

     

  • Email & SMS Alerts

    Email/SMS alert system is one of the key features and gives update information about the submitted manuscript.

    Read more...

  • Article Statastics

    American Journal of PharmTech Research (AJPTR) provides unique facility for authors to know popularity of articles on basis of numbers of PDF download and views. It will display in tabulated format.

  • Online Submission

    American Journal of PharmTech Research (AJPTR) is one of the International open access journal devoted to various disciplines in science and technology.

    Read more...

web counter
web counter