DOI: 10.21276/ajptr
Fri, 19 Apr 2019

Amplification of the Bovine Beta Casein Gene- Relevance to Modern Human Health


Veena Sharma2, Narotam Sharma1, Prem Raj Singh2, Binish Jawed3, Satish Chandra Nautiyal1, R.K Singh1.

1.Molecular Research Laboratory, Department of Biochemistry,Shri  Guru Ram Rai Institute of Medical & Health Sciences, Patel Nagar, Dehradun,(U.K.) India

2.Department of Bioscience and Biotechnology, Banasthali University Banasthali, Rajasthan.

3.Auroprobe Laboratories, Muradnagar, Ghaziabad (U.P), India.



Alleles A1 and A2 of the Bos taurus CSN2 gene are the most common in a number of dairy cattle breeds. A genetic variant of the bovine β-casein gene includes A1 and B which encodes a histidine residue at codon 67, resulting in potential liberation of a bioactive peptide, β-casomorphin, upon digestion. This is an opium family substance, and has been associated with a large number of clinical implications in humans. Study includes amplification of bovine β-casein gene and further differentiation of variants A1 and A2 beta casein in cows. Conventional PCR was done to amplify β-casein gene in three hundred and one specimens. Further eighty amplicons were sent for sequencing. From the sequenced data, 24 were A2 homozygous (A2A2), 11were A1 homozygous (A1A1) and 37 heterozygous (A1A2). Allele discrimination in cows will be significant for the farmers, breeding programmes as well as for dairy industries as the milk variant determination will predict the outcome of the beta casein variants which are of utmost clinical relevance.

Keywords: Cattle, β-Casein, Genetic Polymorphism, Polymerase Chain Reaction, opioid peptides, β-casomorphin.

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