Sat, 18 Nov 2017

A New RP- HPLC Method for the Simultaneous Estimation of Abacavir, Lamivudine and Zidovudine in Tablet Dosage Forms.

 

C.Palavan*1, L.A. Ramaprasad2, J. Varaprasad2, J.V.L.N. Seshagiri Rao1

1. University College of Pharmaceutical Sciences, Andhra University, Visakhapatnam-530003, India.

2. Analytical Development Division, Unit-III, Hetero Drugs Ltd., Hyderabad-500055, India.


 

ABSTRACT

An accurate, precise and reproducible high performance liquid chromatographic method was developed for quantitative estimation of abacavir, lamivudine and zidovudine simultaneously in tablet dosage forms. Separation of the drugs was achieved within 15.0 min on a Hichrom RP-select B column (250 x 4.6 mm; 5µ) by gradient elution using mixtures of 0.02M ammonium acetate and methanol as the mobile phase. The analytes in the eluate were monitored at 250 nm. The retention times obtained for abacavir, lamivudine and zidovudine were 12.172, 1.884 and 4.378 min respectively. The calibration curves were linear over the range of 25-200 µg/mL for abacavir, 12.5-100 µg/mL for lamivudine and 25-200 µg/mL for zidovudine. The performance of the method was validated according to ICH guidelines. The method was found to be suitable for accurate determination of these drugs in tablet dosage forms without any interference from the excipients or endogenous substances.

Key words: Abacavir, Lamivudine, Zidovudine, Determination, HPLC, Gradient elution.


[PDF]  Viewed: 2257  Downloaded: 263
  • Call for paper

    American Journal of PharmTech Research (AJPTR) is a peer-reviewed, bimonthly official international journal allowing access to abstracts and full-text.

    Read more...

     

  • Email & SMS Alerts

    Email/SMS alert system is one of the key features and gives update information about the submitted manuscript.

    Read more...

  • Article Statastics

    American Journal of PharmTech Research (AJPTR) provides unique facility for authors to know popularity of articles on basis of numbers of PDF download and views. It will display in tabulated format.

  • Online Submission

    American Journal of PharmTech Research (AJPTR) is one of the International open access journal devoted to various disciplines in science and technology.

    Read more...

web counter
web counter