Mon, 20 Nov 2017

Multiple shoots regeneration of (anti-cancer plant) Catharanthus roseus - An important medicinal plant

 

Jitendra Mehta1* Deeksha Upadhyay1, Priyanka Paras1, Rukhshar Ansari1, Sunil Rathore1, Shalini Tiwari1

1.Plant Tissue Culture Laboratory and Department of Biotechnology, Vital Biotech Research Institute, University of Kota, Kota- 324009 (Rajasthan), INDIA.


 

ABSTRACT

An efficient and cost effective micropropagation protocol using MS medium developed for Catharanthus roseus, a commercially important medicinal plant. Shootlets were regenerated from nodal explants of stem through axillary shoot proliferation. The induction of multiple shoots from nodal segments were premier in MS medium supplemented with 0.5 mg/l BAP ± 1mg/l NAA. For rooting, different concentration of IBA were used and maximum rooting was recorded on MS medium with 5 mg/l IBA. The rooted plantlets were hardened initially in culture room conditions and then transferred to misthouse.

Keywords: Micropropagation, shoot proliferation, anti-cancer plant

 


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