Mon, 20 Nov 2017

Simultaneous determination of Ceftriaxone and Cefpodoximeproxetil in Commercial Formulations and Spiked Human Plasma using Reversed-Phase High Performance Liquid Chromatography

Jasmin Shah*1, M. Rasul Jan1, Sultan Shah1, Sadaf Durrani1

1. Institute of Chemical Sciences, University of Peshawar, Pakistan


ABSTRACT

A simple and sensitive reversed phase high performance liquid chromatographic (RP-HPLC) method has been developed for the simultaneous quantification of ceftriaxone and cefpodoximeproxetil in artificial mixtures of commercial formulations and spiked human plasma. The separation was carried on C18 (4.6 mm x 250 mm) column with a mixture of acetonitrile and 0.03 M triethylamine (3:1 v/v) adjusted to pH 7.0, with acetic acid, as mobile phase at flow rate of 0.9 mLmin‒1 with UV detector at 240 nm. The chromatographic peaks were recorded at detection wavelength of 240 nm. The retention times of ceftriaxone and cefpodoximeproxetil were 2.111±0.014 min and 4.053±0.013 min respectively. The concentration versus detector response (Peak height) curve is linear in the range of 0.5- 250 µg mL‒1 for ceftriaxone (R2=0.9996), and 0.5-500 µg mL‒1 for cefpodoximeproxetil (R2=0.9995). The limit of detection (3.3σ/S) was found to be 6.62 ng mL‒1 and 14.2 ngmL‒1, respectively, for ceftriaxone and cefpodoximeproxetil. Similarly limit of quantification (10σ/S) was 20 ng mL‒1 for ceftriaxone and 42.9 ngmL‒1 for cefpodoximeproxetil. The developed method was applied to simultaneous determination of these antimicrobials in the artificial mixtures of commercial formulations and spiked human plasma.

Keywords: Ceftriaxone; cefpodoximeproxetil; simultaneous determination; artificial mixture; commercial formulations; spiked human plasma.


[PDF]  Viewed: 1087  Downloaded: 132
  • Call for paper

    American Journal of PharmTech Research (AJPTR) is a peer-reviewed, bimonthly official international journal allowing access to abstracts and full-text.

    Read more...

     

  • Email & SMS Alerts

    Email/SMS alert system is one of the key features and gives update information about the submitted manuscript.

    Read more...

  • Article Statastics

    American Journal of PharmTech Research (AJPTR) provides unique facility for authors to know popularity of articles on basis of numbers of PDF download and views. It will display in tabulated format.

  • Online Submission

    American Journal of PharmTech Research (AJPTR) is one of the International open access journal devoted to various disciplines in science and technology.

    Read more...

web counter
web counter