Mon, 20 Nov 2017

Characterization of Marine streptomyces sp. T1027 Producing β-Carotene Under Light Induction

V. Subhash Chandra Bose1, B. Vishnupriya2, K. Selvam2

1. Bharathiar University Coinbatore-46, Tamilnadu, India

2.Dr.N.G.P. Arts and Science College, Coimbatore-48, Tamilnadu, India.


ABSTRACT

Marine Streptomyces sp.T1027 obtained from South coast of India was discovered to produce and accumulate β-carotene under the influence of light in liquid shake flask culture. The accumulation of β-carotene was growth associated and controlled by light, aeration, carbon and substrate solubility. Starch was the ideal substrate for maximum growth (4.5-6.1 g l-1) and β-carotene accumulation (92-130 μg g-1). Rapid extraction was done in Dimethylsulphoxide, Methanol (1:1) Solvent mixture. The organism was sensitive to a variety of antibiotics; in turn it was able to inhibit the human pathogen Corynebacterium diphtheria. Commercial substrates with high soluble starch content were found to produce maximum biomass and β-carotene production.


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