DOI: 10.21276/ajptr
Thu, 23 May 2019

Development and Validation of RP HPLC Method for Simultaneous Estimation of Ebastine and Montelukast Sodium In Combined Dosage Form

Anand Jigna1*, Mohan Sellappan.2

1.Research scholar, JJT University, Jhunjhunu, Rajasthan, India.

2.Saraswati Institute of Pharmaceutical Sciences, Dhanap, Gandhinagar-382355, India


A specific and accurate reverse phase high performance liquid chromatographic method was developed for the simultaneous determination Ebastine (EBA) and Montelukast Sodium (MONTE) in Combined dosage forms. The analysis was carried out using Phenomenex  C18, column (250 mm × 4.6 mm id, 5 µm particle size) with Mobile phase consisting of  Methanol : Phosphate buffer (65:35 v/v) pH 5.0+0.05 was  pumped at a flow rate was 1.0 ml/ min and  Quantification was achieved with photodiode array (PDA) detection at 261 nm over the concentration range of 10 - 50 µg/mL for ebastine & Montelukast Sodium  with mean recovery of 100.32 ± 0.85 % and 100.15 ± 0.94 % for Ebastine and Montelukast Sodium, respectively. These methods were found to be simple, sensitive, accurate, precise and economical and applicable for the simultaneous determination of Ebastine & Montelukast Sodium in combined dosage form.

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