Sat, 18 Nov 2017

Estimation of Furazolidone In Bulk Drug and Pharmaceutical Dosage Forms by HPLC Method

Bandi Ramachandra1, N.V.S. Naidu1*

1.Department of Chemistry, Sri Venkateswara University, Tirupathi, Andhra Pradesh, India. 517502.


ABSTRACT

A simple, economic, selective, precise, and accurate High Performance liquid Chromatographic method for the analysis of Furazolidone in bulk drug and pharmaceutical formulations was developed and validated in the present study. The mobile phase consists of a mixture of potassium dihydrogen phosphate and acetonitrile 80:20. And Adjust pH 6.50±0.1 with dilute potassium hydroxide solution. This was found to give a sharp peak of Furazolidone at a retention time of 4.245 min. HPLC analysis of Furazolidone was carried out at a wavelength of 354 nm with a flow rate of 1.0 mL/min. The linear regression analysis data for the calibration curve showed a good linear relationship with a regression coefficient (r2) of 1 in the concentration range of 25 to 150 µg ml-1. The linear regression equation was y =46002x+40407. The developed method was employed with a high degree of precision and accuracy for the analysis of Furazolidone. The developed method was validated for accuracy, precision, robustness, detection and quantification limits as per the ICH guidelines.  The wide linearity range, accuracy, sensitivity, short retention time and composition of the mobile phase indicated that this method is better for the quantification of Furazolidone.

Keywords: Furazolidone, HPLC, Validation.


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