Sat, 18 Nov 2017

Bioactive L-asparaginase production by E.coli strains

P. Prema*1, J. Cinthuja2, A. R. Arthi1

1.Research Department of Zoology, V.H.N.S.N.College (Autonomous), Virudhunagar-626 001, Tamilnadu, India.

2. Research Department of Microbiology, V.H.N.S.N.College (Autonomous),Virudhunagar-626 001, Tamilnadu, India


ABSTRACT

L-asparaginase is an anticancer agent, especially for acute lymphoblastic leukemia. Nine E.coli strains were screened forits ability to produce an extracellular L-asparaginase enzyme. The optimum culture conditions for L-asparaginase production was found at pH 8.0, Incubation time 48 h, and 37oC temperature. Highest yields of L-asparaginase(140.5 and 96.4 IU/ml) by E.coli strains using glucose and beef extract as sole carbon and nitrogen sources respectively. 0.6 fold of higher L-asparaginase activity (168.4 IU/ml) was recorded using pUC18 UV60 than the parent strain. 0.54 fold increased L-asparaginase activity (220.6 IU/ml)was observed using pUC18 NTG90.The molecular mass of L-asparaginase was determined by SDS-PAGE and it was found to be 29KDa.   

Keyword: L-asparaginase, E.coli, UV, NTG and SDS-PAGE


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