Mon, 20 Nov 2017

Phytochemical Standardization of Cucumis Melo(L).Extract by HPTLC Techniques

Vidya R*, Kalaivani K, Venkatesh R

Department of Biochemistry, Kongunadu Arts and Science College (Autonomous), Coimbatore, Tamil Nadu-641029, India.


ABSTRACT

To elucidate the terpenoid profile of Cucumis melo (L). fruit extract using high performance thin layer chromatography (HPTLC). The ethanolic extract prepared from the fruit of Cucumis melo (L). using Soxhlet apparatus. n-hexane: ethyl acetate (7.2: 2.9) was employed as mobile phase for terpenoids. 3µl of test solution and 2µl of standard solution was loaded as 6mm band length in the 3 x 10 Silica gel 60F254 TLC plate using Hamilton syringe and CAMAG LINOMAT 5 instrument.  HPTLC analysis was done using CAMAG HPTLC system equipped with automatic TLC sampler IV, TLC scanner 3, REPROSTAR 3 with 12 bit CCD camera for photo documentation, winCATS Planer Chromatography software. The Rf value of the different compounds present in the extract was found to 0.06, 0.21 and 0.93 of peak 1, 2 and 3 respectively. Among them, peak 1 was found to be terpenoid compounds. C. melo fruit extract showed the presence of terpenoids and it was confirmed from the chromatogram after derivatization.  HPTLC profile of terpenoids has been chosen here to reveal the diversity existing at biochemical level in C. melo. Such finger printing is useful in differentiating the species from the adulterant and act as a biochemical marker for this medicinally important plant in the pharmaceutical industry and plant systematic studies.

Keywords: HPTLC, Cucumis melo (L), Secondary metabolites, Terpenoids, Ethanol extract.


[PDF]  Viewed: 1408  Downloaded: 152
  • Call for paper

    American Journal of PharmTech Research (AJPTR) is a peer-reviewed, bimonthly official international journal allowing access to abstracts and full-text.

    Read more...

     

  • Email & SMS Alerts

    Email/SMS alert system is one of the key features and gives update information about the submitted manuscript.

    Read more...

  • Article Statastics

    American Journal of PharmTech Research (AJPTR) provides unique facility for authors to know popularity of articles on basis of numbers of PDF download and views. It will display in tabulated format.

  • Online Submission

    American Journal of PharmTech Research (AJPTR) is one of the International open access journal devoted to various disciplines in science and technology.

    Read more...

web counter
web counter