Mon, 20 Nov 2017

DNA Fingerprint in identification ofSecurinega Leucopyrus (willd.)Through rapidmethod Including Root Analysis

Ajmeer1*, C R Harisha1, T.S. Dudhamal1, Gupta1

1.Department of Shalya, I.P.G.T&R.A. Gujarat Ayurved University, Jamnagar, (GJ) – India.


ABSTRACT

Securinega leucopyrus (Willd.) Muell has long been used by the tribes of Sri Lanka traditionally known as ‘Katupila’and also in India as ‘Humri’. Common in scrub jungls, limited to India, Sri Lanka and Burma. DNA fingerprinting for cultivar or varietal identification has become an important tool for genetic identification in plant breeding and germplasm management.Several different PCR based techniques have been developed during the last decade, each with specific advantages and disadvantages. The Random Amplified Polymorphic DNA (RAPD) marker technique is quick, easy and requires no prior sequence information.Pharmacognostical evaluation of leaf and stem of Securinega leucopyrus (Willd.) already established but no research work have been established on its roots and DNA fingerprints; for the first time leaf samples were subjected to fingerprints and studied the pharmacognostical aspects of roots. Pharmacognostical study showed that presence of oil globules, rosette crystals and uni to multiserriate medullary rays. DNA finger prints showed that OPC 6, OPD 8 and OPB 10 showed that merging 2 bright bands and one light band.

Keywords:  Finger prints, Katupila, RAPD, Root, Securinega leucopyrus.


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