Mon, 18 Dec 2017

Phytochemical evaluation and Biomarker isolation from a Poly-herbal formulation

Rajalakshmy MR1, Sindhu A1, Geetha G2 *

1.R & D Divn., The Arya Vaidya Pharmacy (Coimbatore) Ltd., Palakkad, Kerala, India.

2.Dept. of Pharm. Analysis, PSG College of Pharmacy, Peelamedu, Coimbatore, TN. India


ABSTRACT

Plant derived medicines are popularly used nowadays considering their natural origin and low side effects. Phytochemical compounds are responsible for their various biological activities. Qualitative evaluation of such formulations is a challenging task due to high variability of their chemical components. This work aimed at preparing and evaluating a polyherbal anti-stress medicine, formulated using four potent Ayurvedic drugs namely Withania somnifera, Valeriana wallichii, Bacopa monnieri and Piper longum and also isolating a bioactive marker compound from the same.The formulation was prepared as per classical methods of Ayurveda and analysed for physicochemical characters like loss on drying, total ash content, acid insoluble ash, water soluble extractive value and alcohol soluble extractive value by standard methods. Phytochemical analysis was performed for detecting presence of carbohydrates, phenols, flavonoids, tannins, alkaloids, steroids, Terpenoids, glycosides and saponins and for estimating active groups like total phenolic content, flavonoids, tannins and saponins. High Performance Thin Layer Chromatography fingerprint profiling was performed to confirm the presence of various phytocompounds. The biomarker compound was isolated by column chromatography method and characterized by UV-Visible, H1-NMR, C13-NMR and Mass spectroscopy methods. Physicochemical analysis showed presence of about 18% water soluble components and about 14% alcohol soluble components. Preliminary phytochemical analysis confirmed presence of carbohydrate, phenols, flavonoids, tannins, alkaloids, steroids, glycosides, phytosterols and saponins. Quantitative analysis showed presence of about 7.6% phenols, 1.3% flavonoids, 1.1% tannins and 3.5% saponins. Spectroscopic study indicated isolated compound is a phenolic compound 3, 4, 5-tri hydroxybenzoic acid.

Keywords: Antioxidants, bioactive compound, phenolic compound, flavonoid, memory, anti-stress, Withania somnifera, Valeriana wallichii, Bacopa monnieri, Piper longum


[PDF]  Viewed: 1552  Downloaded: 273
  • Call for paper

    American Journal of PharmTech Research (AJPTR) is a peer-reviewed, bimonthly official international journal allowing access to abstracts and full-text.

    Read more...

     

  • Email & SMS Alerts

    Email/SMS alert system is one of the key features and gives update information about the submitted manuscript.

    Read more...

  • Article Statastics

    American Journal of PharmTech Research (AJPTR) provides unique facility for authors to know popularity of articles on basis of numbers of PDF download and views. It will display in tabulated format.

  • Online Submission

    American Journal of PharmTech Research (AJPTR) is one of the International open access journal devoted to various disciplines in science and technology.

    Read more...

web counter
web counter