DOI: 10.21276/ajptr
Mon, 27 May 2019

Development and Validation of HPTLC and RP-HPLC Methods for the Estimation of Berberine in Coscinium Fenestratum Extract and its Formulation

Jayaprakasam. R1* T.K. Ravi1

1.Department of Pharmaceutical Analysis, College of Pharmacy, Sri Ramakrishna Institute of Paramedical Sciences, 395,Sarojini Naidu Road, Coimbatore -641 044, India


Two simple, sensitive and precise HPTLC and RP-HPLC methods were developed for the estimation of berberine from Coscinium fenestratum, and its formulation. For the determination of berberine by HPTLC method, precoated silicagel 60F254 on aluminium sheets and a mobile phase system comprising of n-butanol: glacial acetic acid : water (8:1:1 % v/v/v ) was selected. After development the plate was scanned and quantified at 350 nm. Linearity was found in the concentration range of 10 to 50 ng/spot (r=0.9992). Limit of detection was found to be 5 ng/spot and limit of quantification was found to be 10 ng/spot. In RP-HPLC method, separation was achieved on a Phenomenex, Luna, C18 column (150 x 4.6mm internal diameter, 5µ particle size) using a mobile phase consisting of potassium dihydrogen phosphate (pH - 2.5) (A)  : acetonitrile (B), where B was run in gradient programme (20% for 0.01-20min, 50% for 20-25min, 50% for 25-26min, 20% for 26-30min), at a flow rate of 1ml/min and the elute was monitored at 220nm. The calibration curve was obtained in the range of 100 - 500 µg/mL. The slope, intercept and correlation coefficient values were found to be 57588, 6041and 0.9959, respectively. The method was validated in compliance with ICH guidelines. Low relative standard deviation and good % recovery values of both the methods showed that the developed methods were highly precise, accurate and can be employed for the routine analysis of formulations containing berberine.

Keywords: Coscinium fenestratum, Berberine, HPTLC, RP-HPLC, Frok capsules, Validation.

[PDF]   Viewed: 1855   Downloaded: 211
  • Call for paper

    American Journal of PharmTech Research (AJPTR) is a peer-reviewed, bimonthly official international journal allowing access to abstracts and full-text.



  • Email & SMS Alerts

    Email/SMS alert system is one of the key features and gives update information about the submitted manuscript.


  • Article Statastics

    American Journal of PharmTech Research (AJPTR) provides unique facility for authors to know popularity of articles on basis of numbers of PDF download and views. It will display in tabulated format.

  • Online Submission

    American Journal of PharmTech Research (AJPTR) is one of the International open access journal devoted to various disciplines in science and technology.


web counter
web counter