DOI: 10.21276/ajptr
Mon, 27 May 2019

A Newer validated and stability indicating UPLC Method for the Estimation of Nordette in Tablet Formulation

V. Sreeram1*, Prof.M.V.BasaveswaraRao2, A.V.D.Nagendrakumar3, V.N.V.Kishore4

1.Department of chemistry, A.G.&S.G. Siddhartha College of Arts & Science, Vuyyuru, Krishna (Dt) -521165.A.P.INDIA.

2. Department of Chemistry, Dr.M.R.A.R. Campus, Krishna University, Krishna district, A.P.,INDIA.

3. Department of Chemistry, GITAM University, Visakhapatnam-530 045, Andhra Pradesh, India

4. Department of chemistry, A.G.& S.G.Siddhartha College of Arts & Science, Vuyyuru, Krishna (Dt) - 521165.A.P.INDIA


A simple, selective, linear, precise and accurate UPLC Method was developed and validated for rapid assay of Nordette in tablet Formulation. Isocratic elution at a flow rate of 0.4ml/min was employed on C8 1.7 µm (2.1 mm x 100 mm) Column at ambient temperature40 °C. Injection Volume was found to be 5.0 µl. The mobile phase consisted of Acetonitrile : Water  60:40 v/v which is filter through a 0.2 µm filter The UV detection wavelength was 220nm and 2µl sample was injected. The retention time for Ethinylestradiol, Levonorgestrel is found to be ± 1.4 minutes and ± 2.1 minutes respectively. A linear regression curve was constructed, and the correlation coefficients (R2) and assessment values calculated. The percentage RSD for both Ethinylestradiol, Levonorgestrel was found to be 1.5%.The Accuracy of method ranges between 97.0 – 102.8%.  The method was validated as per the ICH guidelines. The method was successfully applied for routine quality control analysis of pharmaceutical formulation.

Keywords: Nordette, Ethinylestradiol, Levonorgestrel, UPLC, Recovery, Precise.

[PDF]   Viewed: 1275   Downloaded: 85
  • Call for paper

    American Journal of PharmTech Research (AJPTR) is a peer-reviewed, bimonthly official international journal allowing access to abstracts and full-text.



  • Email & SMS Alerts

    Email/SMS alert system is one of the key features and gives update information about the submitted manuscript.


  • Article Statastics

    American Journal of PharmTech Research (AJPTR) provides unique facility for authors to know popularity of articles on basis of numbers of PDF download and views. It will display in tabulated format.

  • Online Submission

    American Journal of PharmTech Research (AJPTR) is one of the International open access journal devoted to various disciplines in science and technology.


web counter
web counter