DOI: 10.21276/ajptr
Thu, 23 May 2019

Screening of Cytotoxicity Potential of Different Extracts of Marsileaminuta

Akhila.S1*, Bindu A R2, Rosemary J2, Bobby S P2

1. KVM College of Pharmacy, Kokothamangalam P.O, Cherthala.

2. Department of Pharmaceutical Sciences, Mahatma Gandhi University, Cheruvandoor Campus, Ettumanoor P.O, Kottayam.


Cancer, a disease characterized by uncontrolled proliferation of cells that have transformed from the normal cells of the body and is one of the major causes of death in developed and developing nations. Presently available treatment method, chemotherapeutic agents, suffers from drawback of toxicity such as bone marrow suppression, alopecia and vomiting. Its not within the reach of common man. Therefore challenging task is to identify quick and novel methods which can be of therapeutic value in human cancers. Plants have a long history and important role as a source of effective anti-cancer agents. Hence in the present study, one of the traditionally used plant, M.minuta was screened for cytotoxicity. Successive solvent extraction was carried out and the petroleum ether, chloroform, ethyl acetate, alcoholic and aqueous extracts of M.minuta were prepared. Extracts were subjected to Brine shrimp lethality assay and it was observed that % mortality increased gradually with the increase in concentration of the test samples. Ethyl acetate and alcoholic extracts which showed better cytotoxicty were further screened for their cytotoxicity towards breast cancer cell lines using MTT cell viability assay. In the same, ethyl acetate extract was found to be significantly cytotoxic towards cell lines compared to that of alcoholic extract which may be due to higher amount of phenolic compounds present in them. The results obtained in this study further supported the traditional usage of the drug and the activity of drug can be attributed to major bioactive phytochemical class; poly phenolics, present in the drug.

Keywords: Marsileaminuta, cytotoxicity, phenolics, MTT assay, Brine shrimp

[PDF]   Viewed: 949   Downloaded: 80
  • Call for paper

    American Journal of PharmTech Research (AJPTR) is a peer-reviewed, bimonthly official international journal allowing access to abstracts and full-text.



  • Email & SMS Alerts

    Email/SMS alert system is one of the key features and gives update information about the submitted manuscript.


  • Article Statastics

    American Journal of PharmTech Research (AJPTR) provides unique facility for authors to know popularity of articles on basis of numbers of PDF download and views. It will display in tabulated format.

  • Online Submission

    American Journal of PharmTech Research (AJPTR) is one of the International open access journal devoted to various disciplines in science and technology.


web counter
web counter