Mon, 18 Dec 2017

Action of Ethyl Extracts of KhayaSenegalensis on Rat Hepatocytes. Evaluation of Toxicity

Assou K. Eudoxie1, Behanzin Justin1, Ahokpe Melanie1, Wouetola Ermence1, Agossou A. Essè1, Sezan Alphonse1*

1 Laboratory of Biomembranes and Cell Signaling University of AbomeyCalavi, 06 BP 3041 Cotonou, Benin.


ABSTRACT

The present work aims to study the effect of ethyl extract stem bark of Khayasenegalensis on the lipid profile of rat hepatocytes. For this, a sub-chronic gavage was performed on batches of 8 Wistar rats received 3 respectively, by oral administration of the extract at doses 2.5 mg / kg; 5mg / Kg; 10mg / Kg; 25mg / Kg; 50mg / Kg; 100mg / kg and 200mg / kg body weight for 14 days (control group received instead of the extract of Khayasenegalensis of distilled water) and isolation of hepatocytes was made after killing rats. Then, all the lipids (phospholipids and neutral) were extracted and separated by thin layer chromatography on two different plates. TLC of lipids (phospholipids and neutral) revealed an appearance of membrane lipids in the dose of 5 mg / kg; 10mg / Kg; 25mg / Kg; 50mg / Kg; 100mg / kg and 200mg / kg compared to control, while in the dose of 2.5 mg / kg there was no significant change compared to the control. The appearance of neutral lipids is only degradation of the phospholipids and the phospholipids provides that Khayasenegalensis lysed membrane of hepatocytes, which leads to the dispersion of the phospholipids. So it was only after dispersion, these lipids have started to deteriorate in neutral lipids. Where the administration of ethyl extract of trunk bark khayasenegalensis high dose may cause necrosis of liver cells, thus at high doses is toxic Khayasenegalensis. And the most efficient to mitigate the risk of toxicity are those doses less than or equal to 2.5 mg / Kg.

Keywords: Khayasenegalensis, phospholipids, neutral lipids, CCM, necrosis.


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