DOI: 10.21276/ajptr
Fri, 22 Mar 2019

RP-HPLC Method development and validation for the Estimation of Etoposide in Bulk Drug and its Pharmaceutical Dosage Form.

B.Lakshmi1*, K.Rama Krishna2, K.N.Jayaveera3, G.V.Padmakar rao4

1. Department of Chemistry, GITAM University, Hyderabad- 502329, INDIA

2. Department of Chemistry, GIS, GITAM University, Visakhapatnam – 530045, AP, INDIA

3. Department of Chemistry, JNTU, Anantapur- 515002, AP, INDIA

4. Aurobindo Research Centre, Hyderabad-500090, India.


A specific and accurate HPLC method is developed for the determination of etoposidein bulk drugs and in solid capsule dosage form. Best symmetric peak shape obtained with Inertsil ODS C-18 column (250 X 4.6 mm, 5μ) column in an isocratic mode, with retention time 5 min.The mobile phase used was Water : Acetonitrile  60:40(v/v)with flow rate 1.0 ml/min and effluent was monitored at 263 nm. As per ICH guidelines method has validated. Method has found linear in the range of 5-45 µg/ml. The LOD and LOQ were found to be 0.02 and 0.06 µg/ml respectively. Method was found specific with respective of diluents, excipients and degradants.

Keywords: Etoposide, RP-HPLC, ICH guidelines, Method validation.

[PDF]   Viewed: 1231   Downloaded: 126
  • Call for paper

    American Journal of PharmTech Research (AJPTR) is a peer-reviewed, bimonthly official international journal allowing access to abstracts and full-text.



  • Email & SMS Alerts

    Email/SMS alert system is one of the key features and gives update information about the submitted manuscript.


  • Article Statastics

    American Journal of PharmTech Research (AJPTR) provides unique facility for authors to know popularity of articles on basis of numbers of PDF download and views. It will display in tabulated format.

  • Online Submission

    American Journal of PharmTech Research (AJPTR) is one of the International open access journal devoted to various disciplines in science and technology.


web counter
web counter