Mon, 18 Dec 2017

Biochemical Changes During Rhizogenesis in Callus Cultures of Tribulus Terrestris Linn

Singh M1, Goyal SC1, Gulati D1*

1. Department of Botany and Plant Physiology, Chaudhary Charan Singh Haryana Agricultural University, Hisar 125004, Haryana, India.


ABSTRACT

Biochemical changes during in vitro root formation in Tribulus terrestris were investigated in the callus derived from nodal explants on Murashige and Skoog’s (MS) medium supplemented with 1.0 mg/l 2,4-D+ 0.5 mg/l Kinetin. The callus maintained on MS medium fortified with 2.0 mg/l 2, 4-dichlorophenoxy acetic acid (2,4-D) + 0.5 mg/l Kinetin. The above callus subcultured on Murashige and Skoog’s (MS) medium + 2.0 mg/l α-naphthalene acetic acid (NAA). Metabolites like starch and total; soluble sugars decreased while reducing sugar, total phenol and total protein increased during root differentiation in callus. Enzyme activity viz. α-amylase, acid invertase, peroxidase and acid phosphatase increased during root differentiation.

Keywords: Tribulus terrestris Linn, Biochemical changes, Rhizogenesis, Callus.


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