DOI: 10.21276/ajptr
Sat, 25 May 2019

A UV Spectrophotometric Method for the Determination of Methotrexate in Pharmaceutical Dosage Form

Maroti B. Shelke*1, Faijal E. Patel1, Shivani U. Gadve1, Pratiksha B. Shinde1, Mukesh T. Mohite1

1. Padmashree Dr. D. Y. Patil College of Pharmacy, Akurdi, Pune - 411044, Maharashtra, India


Two simple, precise and economical UV methods have been developed for estimation of Methotrexate in bulk formulation. Method A involves measurement of UV absorbance in Zeroorder derivative at 303nm. Method B deals with area under curve measurement (AUC method), which involves the calculation of integrated value of absorbance with respect to wavelength between 300-306nm.  The drug follows Beer-Lambert’s law in the concentration range of 3-15μg/ml in both the methods. Results of analysis were validated statistically and were found to be satisfactory. Thus proposed methods can be successfully applied for estimation of Methotrexatein routine analytical work.

Keywords:Methotrexate, Zero order derivatives, Area under Curve method (AUC), UV Spectrophotometer.

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