Mon, 18 Dec 2017

In vitro Applications of In Silico Designed Antibiofilm Agents for Staphylococcus epidermidis

Aymen F. Al-Mulla1, Zahra M. Al-Khafaji1

1. Institute of Genetic Engineering and Biotechnology for Postgraduate Studies / Univ. of Baghdad, IRAQ.

 


ABSTRACT

In recent years anti-virulence agents have been used widely to reduce bacterial resistance and prevent damage to host cells and normal flora.  Six  molecules were used in this study (according to previous in silico studies) to detect their antibiofilm activity in lab. Four molecules gave positive results and were as follows : Acetaminophen inhibited biofilm production 100% at 11000µg/ml concentration, Acetylsalicylic Acid inhibition was 100% at 1600µg/ml , Ibuprofen inhibited 35% of biofilm production at 600µg/ml and Acetic Acid inhibition was 25% at 1000 µg/ml concentration .All the molecules at the used concentrations were found to affect biofilm production without significant change in bacterial growth. It was concluded that a structure based drug design strategy using Ligand Based Virtual Screening had a success score of about 60% and that Acetaminophen, Acetylsalicylic Acid, Ibuprofen and Acetic Acid can be used as antibiofilm molecules . Also Non-Steroidal Anti-Inflammatory Drugs family can be a useful library for antibiofilm future investigations.

Keywords: Drug design, antibiofilm, S. epidermidis, biofilm, acetaminophen, acetic acid, acetylsalicylic acid, ibuprofen.

 


[PDF]  Viewed: 737  Downloaded: 126
  • Call for paper

    American Journal of PharmTech Research (AJPTR) is a peer-reviewed, bimonthly official international journal allowing access to abstracts and full-text.

    Read more...

     

  • Email & SMS Alerts

    Email/SMS alert system is one of the key features and gives update information about the submitted manuscript.

    Read more...

  • Article Statastics

    American Journal of PharmTech Research (AJPTR) provides unique facility for authors to know popularity of articles on basis of numbers of PDF download and views. It will display in tabulated format.

  • Online Submission

    American Journal of PharmTech Research (AJPTR) is one of the International open access journal devoted to various disciplines in science and technology.

    Read more...

web counter
web counter