Mon, 18 Dec 2017

Simultaneous Estimation of Gentamicin and Clobetasol in Bulk and Pharmaceutical Formulations by RP-HPLC Method

Iffath Rizwana1, K. Vanitha Prakash2*, G. Krishna Mohan3

1. Research Scholar, R & D, Jawaharlal Nehru Technological University Kakinada, Kakinada, A. P. India. & Dept. of Pharmaceutical Analysis, Deccan School of Pharmacy, Hyderabad, A. P. India.

2. Department of Pharmaceutical Analysis, SSJ College of Pharmacy, Gandipet, Hyderabad, A.P. India.

3. Centre for Pharmaceutical Sciences, IST, JNTU Hyderabad, A.P. India.


ABSTRACT

A new simple, precise, accurate and reproducible RP-HPLC method for simultaneous estimation of gentamicin and clobetasol in bulk and pharmaceutical formulations. Separation of gentamicin and clobetasol was successfully achieved on a Zorbax C18 (150 mm x 4.6mm x 5µ ) in an isocratic mode utilizing disodium hydrogen phosphate buffer and methanol (60:40 v/v) at a flow rate of 1.0 mL/min. The method was validated according to ICH guidelines for linearity, sensitivity, accuracy, precision, specificity and robustness. The response was found to be linear in the drug concentration range of 0.1-0.30 mg/mL for gentamicin and 0.05–0.15 mg/mL for clobetasol. The correlation coefficient was found to be 0.9997 for both the drugs. The LOD and LOQ for gentamicin were found to be 0.3525 µg/mL and 1.1751 µg/mL respectively. The LOD and LOQ for clobetasol were found to be 0.1938 µg/mL and 0.6460 µg/mL respectively.  The proposed method was found to be good percentage recovery for gentamicin and clobetasol, which indicates that the proposed method is highly accurate. The specificity of the method shows good correlation between retention times of standard solution with the sample solution. Therefore, the proposed method specifically determines the analyte in the sample without interference from excipients of pharmaceutical dosage forms.

Keywords: RP-HPLC, gentamicin, clobetasol, pharmaceutical formulation, analysis.


[PDF]  Viewed: 590  Downloaded: 88
  • Call for paper

    American Journal of PharmTech Research (AJPTR) is a peer-reviewed, bimonthly official international journal allowing access to abstracts and full-text.

    Read more...

     

  • Email & SMS Alerts

    Email/SMS alert system is one of the key features and gives update information about the submitted manuscript.

    Read more...

  • Article Statastics

    American Journal of PharmTech Research (AJPTR) provides unique facility for authors to know popularity of articles on basis of numbers of PDF download and views. It will display in tabulated format.

  • Online Submission

    American Journal of PharmTech Research (AJPTR) is one of the International open access journal devoted to various disciplines in science and technology.

    Read more...

web counter
web counter