DOI: 10.21276/ajptr
Thu, 23 May 2019

Spectroscopic Method for the Determination of Drugs Containing Phenol Group by Using 2, 4- Dinitro Phenylhydrazine

Roopa  Kothathi Papanna1*, Jayanna bidarur Krishne gowda2, Padmarajaiah Nagaraja3

1. R & D centre, Bharathiar university, Coimbatore - Tamilnadu.

2. Department of chemistry, B.N.M. Institute of technology, Bangalore.

3. Department of studies in chemistry, University of Mysore, Manasagangotri Mysore.


A spectroscopic method has been proposed for the determination of two phenolic drugs; Phenylephrine hydrochloride and Pyridoxine hydrochloride. The method is based on the oxidation of 2, 4- Dinitro phenylhydrazine and coupling of the oxidized product with drugs to give intensely colored chromogen. Under the proposed optical condition, Beer’s law was obeyed in the concentration range of 2.5 - 30 µg mL-1and 5 – 20 µg mL-1 for Phenylephrine Hydrochloride and Pyridoxine Hydrochloride respectively. The limit of detection (LOD) and limit of quantification (LOQ) were 0.3, 1.95 µg mL-1 and 0.95, 0.64 µg mL-1 in the same order. No interference was observed from common pharmaceutical adjuvants. The suggested method was further applied for the determination of drugs in commercial pharmaceutical dosage forms, which was compared statistically with reference methods by means of t- test and F- test and were found not to differ significantly at 95% confidence level. The procedure is characterized by its simplicity with accuracy and precision.

Keywords: 2, 4- Dinitro phenylhydrazine, Phenylephrine hydrochloride, Pyridoxine hydrochloride and Spectrophotometry.

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