Mon, 18 Dec 2017

Development and Validation of Stability Indicating UV-VIS Spectrophotometric Method for Estimation of Acyclovir in Bulk Drug and Pharmaceutical Dosage Form

Arun Kumar Teotia1*, Suvarna S.Vanjari1, Rajendra B. Patil1, K. R. Khandelwal1

1. Department of Pharmaceutical Analysis, JSPM’S Rajarshi Shahu College of Pharmacy and Research, Tathawade, Pune-33.


ABSTRACT

The simple, accurate, precise, sensitive, specific and cost- effective UV-VIS Spectrophotometric method was developed for the estimation of acyclovir in bulk and pharmaceutical dosage form, used as an antiviral drug. The absorption maxima of the drug were found to be 252.6nm. in 0.1N H2SO4solvent. The method was validated as per the ICH Guidelines. A linear response was observed in the range of 2-10µg/ml with a regression coefficient of 0.999. The developed method was precise; the % RSD was found to be less than 2%. The LOD & LOQ were found to be 2.3 and 7.0µg/ml respectively. The standard drug was subjected to acidic, alkaline, oxidative, thermal & photolytic stress degradation conditions. The samples so generated were used for degradation studies using the stability indicating UV-Visible Spectrophotometric method. This method was used for the determination of Acyclovir in tablet and cream formulation without interferences of the excipients, this indicated specificity of method.

Keywords: Acyclovir, stress degradation conditions, UV-VIS Spectrophotometer, ICH guidelines- validation.


[PDF]  Viewed: 616  Downloaded: 107
  • Call for paper

    American Journal of PharmTech Research (AJPTR) is a peer-reviewed, bimonthly official international journal allowing access to abstracts and full-text.

    Read more...

     

  • Email & SMS Alerts

    Email/SMS alert system is one of the key features and gives update information about the submitted manuscript.

    Read more...

  • Article Statastics

    American Journal of PharmTech Research (AJPTR) provides unique facility for authors to know popularity of articles on basis of numbers of PDF download and views. It will display in tabulated format.

  • Online Submission

    American Journal of PharmTech Research (AJPTR) is one of the International open access journal devoted to various disciplines in science and technology.

    Read more...

web counter
web counter