Sat, 16 Dec 2017

Isolation, Screening and Characterization of Marine Actinomycetes for Novel Bioactive Compounds

Mantripragada Visalakshi1*, Veerendra Kumar Bapatla1

1. Department of Biotechnology, GITAM Institute of Science, GITAM University, Visakhapatnam, India.


ABSTRACT

The present study deals with the isolation, screening and characterization of the actinomycetes from the marine sediments, which were collected from different locations of Bay of Bengal. Selective media and the pre-treatment strategies enhanced the isolation and screening of novel marine actinomycetes. A total of 8 marine samples were collected. The pre-heat treatment method and a combination of 4 enrichment media were found to be effective in selectively isolating marine actinomycetes. The top six potent isolates were subjected to detailed morphological, cultural, biochemical and physiological characterization. A total of 63 isolates were isolated. The antimicrobial activity was studied for all the 63 isolates. The preliminary study of 63 isolates for antimicrobial activity by cross streak method indicated that 42 isolates had antagonistic properties. All these 42 isolates were subjected to submerged fermentation studies. It was observed that 12 isolates exhibited antibacterial activity, 6 isolates showed antifungal activity whereas 6 isolates exhibited both antibacterial and antifungal activities. The present study was an attempt to use different methods to screen, select and isolate marine actinomycetes from the sediments of Bay of Bengal with intrinsic antimicrobial activity against a variety of microbial pathogens.

Keywords: Marine actinomycetes, Antimicrobial activity, Bioactive compounds.


[PDF]  Viewed: 1110  Downloaded: 214
  • Call for paper

    American Journal of PharmTech Research (AJPTR) is a peer-reviewed, bimonthly official international journal allowing access to abstracts and full-text.

    Read more...

     

  • Email & SMS Alerts

    Email/SMS alert system is one of the key features and gives update information about the submitted manuscript.

    Read more...

  • Article Statastics

    American Journal of PharmTech Research (AJPTR) provides unique facility for authors to know popularity of articles on basis of numbers of PDF download and views. It will display in tabulated format.

  • Online Submission

    American Journal of PharmTech Research (AJPTR) is one of the International open access journal devoted to various disciplines in science and technology.

    Read more...

web counter
web counter