Sat, 16 Dec 2017

Removal of Phorbol Ester from Jatropha Curcas Seed Cake Reduces Toxicity in Rats Blood Plasma Studied through Various Parameters

Amit Kumar Sharma1, Shivani Srivastava1, Akhoury Sudhir Kumar Sinha2, Yamini Bhushan Tripathi1*

1. Department of Medicinal Chemistry, Institute of Medical Sciences, Banaras Hindu University, Varanasi 21005, UP India.

3. Department of Chemical Engg, .I.I T., Banaras Hindu University, Vanarasi-221005. UP India.


ABSTRACT

Jatropha curcas in spite of having many medicinal effects is not used usually as medicine because of the presence of toxic components like phorbol ester. In this study, we have compared many chemical and biochemical parameters between plant extract (MEMJC) Methanolic extract of mechanical cake of Jatropha curcas seeds and without the presence of phorbol ester (MEHJCAT). Alkali treated mechanical cake of Jatropha curcas seeds .The phorbol ester was removed by alkali treatment. Phytochemical analyses were done to determine composition. The survival study was evaluated using Kaplan Meier Chart. SOD activity was assayed by the method of McCord and Fridovich and Catalase activity was assayed by Aebi’s method. Urea, Creatinine, triglyceride level in plasma was measured using their respective kits. The removal of phorbol ester through alkali treatment makes the MEHJCAT less toxic or less vulnerable to generate free radical in rats. Through oral fat tolerance test, we have found that MAHJCAT reduces the absorption of triglyceride in blood. The results of renal function test also favored MEMJC as more toxic then MEHJCAT. Also from kaplen meier survival curve, it has been found that both drugs MEMJC and MEHJCAT have no mortality significance proved by the measurement of LD50. So, we have concluded that MEHJCAT as better medicinal plant product through the study of all biochemical parameters.

Keywords: Jatropha curcas seed cake, Oral fat tolerance test, Hyperglycemic, Phytochemicals.


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