Sat, 16 Dec 2017

In Vitro Enzyme Inhibition and Thin Layer Chromatography Profiling of Leaf Extract of Phyllanthus Emblica Linn against Type-Ii Diabetes

Jayashree Dutta1*, Sharmistha Saikia1, Mohan Chandra Kalita1

1. Department of Biotechnology, Gauhati University, 781 014, Guwahati, Assam.


ABSTRACT

Phyllanthus emblica Linn commonly known as Indian gooseberry is one of the most important plants used in Ayurveda, Unani and Siddha. In the present study, the leaf methanol extract of P. emblica was studied against the carbohydrate metabolizing enzyme alpha amylase and alpha glucosidase. P. emblica leaf methanol extract showed a moderate α – amylase inhibitory activity (IC50 – 155. 10 ± 0.91µg/ml) and significantly high (P<0.05) α - glucosidase inhibitory activity (IC50 – 141.99 ± 0.43 µg/ml) compared to the standard drug acarbose (IC50 – 254.28 ± 0.88 µg/ml).The kinetics study of the leaf methanol extract of P. emblica showed uncompetitive mode of inhibition for both the enzymes. Phytochemical screening reveals the presence of several bioactive groups like phenol/tannin, flavanoid, alkaloid, saponin and terpenoids. The TLC fingerprinting confirms the presence of bio molecules like quercetin and gallic acid in the crude methanol extract of P. emblica leaf. The study conclude that  presence of these bioactive groups including quercetin and gallic acid, contribute towards the inhibition of carbohydrate metabolizing enzymes like alpha amylase and alpha glucosidase.

Keywords: Post prandial hyperglycemia, α- amylase, α- glucosidase, flavonoids, phenol and thin layer chromatography.


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