Tue, 21 Nov 2017

Stability indicating RP-HPLC method for the simultaneous determination of Spironolactone and Hydroflumethiazide

Katta Suryaprabha1, M.Subbarao1 ,T. Neeharika, Golkonda Ramu1, 2, C.Rambabu1*

1.Acharya Nagarjuna University, Nagarjuna Nagar, Guntur, Andhra Pradesh, India

2.Sir C. R. Reddy College, P.G .Courses, Eluru, West Godavari, Andhra Pradesh, India


ABSTRACT

A stability indicating RP-HPLC method was developed for the simultaneous determination of Spironolactone (SRL) and Hydroflumethiazide (HFM) in pharmaceutical dosage form. Inertsil ODS - C18 (250 mm x 4.6 mm, 5 µm) column and mobile phase of methanol: acetonitrile : phosphate buffer in the ratio of 55:40:05 v/v at a flow rate of 1.0 mL/min was used for separation of the components. The components were detected at a wavelength of 221nm using UV detector. The Spironolactone and Hydroflumethiazide were separated at retention time 4.67 and 6.74 min respectively. The developed method was validated in terms of precision, accuracy, linearity, specificity, limit of detection, limit of quantitation. The range of linearity was found to be 5-30 µg/mL for Hydroflumethiazide and 5-30 µg/mL for Spironolactone. The proposed method was applied to study the stability of the drugs under different degradation conditions such as acid, alkali, peroxide, thermal and photo light. The developed method was found to be simple, sensitive and rapid and hence, It can be adopted in any laboratory for quality control analysis.

Keywords: Spironolactone , Hydroflumethiazide, quality control, stability, validation.


[PDF]  Viewed: 196  Downloaded: 52
  • Call for paper

    American Journal of PharmTech Research (AJPTR) is a peer-reviewed, bimonthly official international journal allowing access to abstracts and full-text.

    Read more...

     

  • Email & SMS Alerts

    Email/SMS alert system is one of the key features and gives update information about the submitted manuscript.

    Read more...

  • Article Statastics

    American Journal of PharmTech Research (AJPTR) provides unique facility for authors to know popularity of articles on basis of numbers of PDF download and views. It will display in tabulated format.

  • Online Submission

    American Journal of PharmTech Research (AJPTR) is one of the International open access journal devoted to various disciplines in science and technology.

    Read more...

web counter
web counter