DOI: 10.21276/ajptr
Sun, 24 Feb 2019

UV Visible Spectrophotometric Estimation of Antibiotic Drugs

C.M.Bhaskar Reddy1*, Dr G.V. Subba Reddy 2, Dr N.Ananda Kumar Reddy 3

1. Research Scholar ,Dept of chemistry ,Rayalaseema University ,Kurnool ,AP, India.

2. Jawaharlal Nehru Technological University Anantapur College of Engineering (Autonomous),Pulivendula,Kadapa (dist),AP , India 516 390

3.S.V. Degree  College,Kadapa, kadapa (dist),AP , India


The main aim of current study was to develop U V visible spectrophotometric method for the quantitative determination of Flucloxacillin and  Ceftriaxone in bulk and  pharmaceutical formulations. Flucloxacillin  is a  broad spectrum beta -lactam antibiotic, belonging to the isoxazolyl family of penicillin’s. Ceftriaxone is a semi synthetic, broad-spectrum and third generation  cephalosporin antibiotic for intravenous or intramuscular administration. The solvents used in this method is chloroform ,double distilled water (1:1) in presence of phosphate buffer of pH 7.4. Linearity test solutions for the assay method were prepared in the range of 2-12 μg/ mL and  4-16 μg/mL respectively for determination of flucloxacillin and ceftriaxone which obeyed Beer’s law and found to linear. From the experimental results reveal that absorption maximum of 270 nm for flucloxacillin and 290 nm for ceftriaxone were found. Validation of the developed method for two drugs was carried out as per ICH requirements.

Keywords: Flucloxacillin, ceftrixone, U v visible spectrophotometry, Beer’s law, validation.


[PDF]   Viewed: 622   Downloaded: 76
  • Call for paper

    American Journal of PharmTech Research (AJPTR) is a peer-reviewed, bimonthly official international journal allowing access to abstracts and full-text.



  • Email & SMS Alerts

    Email/SMS alert system is one of the key features and gives update information about the submitted manuscript.


  • Article Statastics

    American Journal of PharmTech Research (AJPTR) provides unique facility for authors to know popularity of articles on basis of numbers of PDF download and views. It will display in tabulated format.

  • Online Submission

    American Journal of PharmTech Research (AJPTR) is one of the International open access journal devoted to various disciplines in science and technology.


web counter
web counter