Tue, 21 Nov 2017

Standardization, Phytochemical Screening and TLC Profiling of Polygonum Glabrum

Raja S*1, Ramya I1

1.GITAM Institute of Pharmacy, GITAM University, Visakhapatnam- Andhra Pradesh.


ABSTRACT

Standardization of medicinal plants is an important step as it determines the identity and quality of a plant. The aim of present study was to standardize Polygonum glabrum by evaluating physicochemical parameters. Successive extraction, phytochemical screening and thin layer chromatography analysis were studied to detect the presence of various phytoconstituents. Physicochemical parameters such as loss on drying, total ash, acid insoluble ash, water soluble extractive and alcohol soluble extractive values were found to be 06.7±1.19%w/w, 15.52±0.28%w/w, 4.65±0.02%w/w, 20.05±1.07%w/w and 13.66±0.20%w/w, respectively. In phytochemical screening, petroleum ether extract revealed the presence of steroids, tannins and saponins whereas chloroform extract confirmed the presence of alkaloids, flavonoids and carbohydrates. Ethylacetate extract showed the presence of phenolic compounds, steroids, proteins and glycosides. Further, major constituents like alkaloids, sugars, phenols, proteins, steroids, saponins, tannins and flavonoids were present in methanol extract. In addition, thin layer chromatography analysis supports the presence of terpenoids, alkaloids and flavonoids in petroleum ether, chloroform and methanol extracts, respectively.  It can be concluded that the standardization parameters investigated in this study could assist in safe, accurately collection, handling of crude materials, ensure efficacy and stability of ended product. Further, the major active constituents were identified in different extracts of Polygonum glabrum

Keywords: Polygonum glabrum, Physicochemical analysis, Standardization, Thin layer chromatography


[PDF]  Viewed: 308  Downloaded: 74
  • Call for paper

    American Journal of PharmTech Research (AJPTR) is a peer-reviewed, bimonthly official international journal allowing access to abstracts and full-text.

    Read more...

     

  • Email & SMS Alerts

    Email/SMS alert system is one of the key features and gives update information about the submitted manuscript.

    Read more...

  • Article Statastics

    American Journal of PharmTech Research (AJPTR) provides unique facility for authors to know popularity of articles on basis of numbers of PDF download and views. It will display in tabulated format.

  • Online Submission

    American Journal of PharmTech Research (AJPTR) is one of the International open access journal devoted to various disciplines in science and technology.

    Read more...

web counter
web counter