Tue, 21 Nov 2017

A Novel UV-Spectrophotometric Method Development and Validation of Dolutegravir In Bulk and Its Laboratory Synthetic Mixture

SK.Masthanamma1*,  K.Vasundhara1,  S.Srilakshmi1,  SK.Fathimabi1

1.Department  of  Pharmaceutical  Analysis,  University  College  of  Pharmaceutical  Sciences,  Acharya  Nagarjuna  University,  Nagarjunanagar,  Guntur–522510,  Andhra  Pradesh


ABSTRACT

Present  study  describes  the  spectrophotometric  method  development  and  subsequent  validation  of  dolutegravir  with  greater  precision  and  accuracy.  AIDS  is  the  most  dreadful  disease  in  the  society;  this  has  a  very  high  mortal  rate  among  the  countries,  as  results  loosing  many  beneficial  personalities.  There  are  many  antiviral  and  antiretroviral  drugs,  brought  forward  by  many  efficient  scientists. A  simple,  accurate,  novel,  safe,  and  precise  method  could  be  developed  for  the  estimation  of  Dolutegravir.  Spectrophotometric  measurements  were  carried  out  using  Schimadzu    double  beam(UV-1800  model)  Ultra  violet  visible  spectrophotometer  with  10mm  matched  quartz  cells  and  water  as  solvent. Linearity  for  the  method  was  found  in  the  range  of  2-14μg/ml  (r2=0.997).  Tablet  formulation  was  analyzed  and  %  assay  for  the  absorption  maxima  was  found  to  be  95.6%. The proposed method was validated as  per  ICH  guidelines.  Validated  studies  demonstrated  that  proposed  method  is  simple,  accurate,  precise,  specific,  rapid,  reliable,  and  reproducible.

Keywords:  Dolutegravir, spectroscopy, dosage form, validation.  


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