Tue, 21 Nov 2017

In Vitro Cytotoxic Activity on Root Extracts and Fractions of Jatropha Gossypiifolia Linn

R. Pandiyan1, M. Sathish1*, A. Jerad Suresh1

1. Department of Pharmaceutical Chemistry, College of Pharmacy, Madras Medical College, Chennai-600003.


ABSTRACT

The present study was to evaluate preliminary phytochemical analysis and in vitro cytotoxic activity on root extracts and fractions of Jatropha gossipiifolia (family Euphorbiaceae). The Chloroform and ethanol extract was obtained by hot continuous percolation by soxhlet apparatus. The phytochemical analysis of both the extracts shows the presence of flavonoids, alkaloids, tannins, phenolic compounds and steroids. Both the extracts subjected to in vitro cytotoxic activity against breast cancer cell line (MCF–7) cell line by MTT assay. The active extract (ethanol) was then subjected to fractionation by column chromatography by gradient elution from n hexane – ethanol. The fractions (chloroform 100%, chloroform: ethyl acetate, ethyl acetate: ethanol, ethanol 100%) The fractions were then subjected to in vitro cytotoxic activity against MCF– 7 cell line by MTT assay. The ethanol extract shows good anticancer activity. The percentage growth of MCF-7 cells in chloroform and ethanol extract treated against MCF-7 cell line was found to be 49.59 and 28.46 respectively. The EC50 value of chloroform and ethanol extract was found to be 0.00018 & 0.00055mg/ml respectively. The ethanol 100% shows good anticancer activity compared to other fractions against MCF-7 cell line with the percentage growth of 14.650 and EC50 value was found to be 0.00087mg/ml.

Keywords: Jatropha Gossypiifolia, MTT assay, MCF–7 cell line, Column chromatography


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